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revert 700 total protein stain kit  (LI-COR)


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    Structured Review

    LI-COR revert 700 total protein stain kit
    Revert 700 Total Protein Stain Kit, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 2152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/revert+700+total+protein+stain+kit/REVERT+700+TOTAL+PROTEIN+STAIN+KIT/custom%40926-11016%4042389555
    Average 96 stars, based on 2152 article reviews
    revert 700 total protein stain kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis
    Article Snippet: .. The blots were then stained for total protein using the Revert 700 Total Protein Stain Kit from LI-COR Biotech (Cat: 926-11016) according to the manufacturer’s protocol. .. After removing the Total Protein Stain, blots were blocked for 1 h in LI-COR Biotech In- https://doi.org/10.3390/cells15080671 tercept Blocking Buffer (Cat: 927-60001) and probed with primary antibodies overnight at 4 ◦C.

    Article Title: Jagged1 regulates extracellular matrix deposition and remodeling in triple-negative breast cancer
    Article Snippet: .. Cell lysates were directly spotted on a nitrocellulose membrane, allowed to dry, rinsed with MQ-H 2 O, and stained for total protein using the Revert 700 Total Protein Stain Kit (Li-COR) according to the manufacturer’s instructions. ..

    Article Title: Tissue and Isoform-Specific Effects of Platelet-Derived Growth Factor on Neonatal-Derived Dermal and Fetal-Derived Lung Fibroblast Profibrotic Functions
    Article Snippet: Approximately 7.5 μg of protein was loaded per well in a 4–20% Mini-PROTEAN TGX Precast Protein Gel (cat. 4561096, Bio-Rad, Hercules, CA, USA). .. Protein was transferred to a 0.45 μm nitrocellulose membrane (cat. 1620115, Bio-Rad) and total protein was quantified with the Revert 700 Total Protein Stain Kit (cat. 926-11016, LI-COR Biotech), following the manufacturer’s instructions. .. Membranes were blocked for one hour at room temperature with Intercept Phosphate-Buffered Saline (PBS) Blocking Buffer (cat. 927-70001, LI-COR Biotech) and probed overnight at 4 °C with anti-collagen-I antibody (1:1000 dilution, clone EPR7785, cat. ab138492, Abcam, Waltham, MA, USA), anti-fibronectin antibody (1:2000 dilution, clone IST-9, cat. sc-59826, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-smooth muscle actin polyclonal antibody (1:300 dilution, cat. PA5-85070, Thermo Fisher Scientific), anti-PDGFR alpha antibody (1:1000 dilution, clone EPR22059-270, cat. ab203491, Abcam), anti-PDGFR beta antibody (1:1000 dilution, clone 42G12, cat. ab69506, Abcam), anti-ERK1/ERK2 antibody (1:500 dilution, clone ERK-7D8, cat. 13-6200, Thermo Fisher Scientific), or anti-phospho-ERK1/2 antibody (1:1000 dilution, clone 2P8Q2, cat. MA5-38228, Thermo Fisher Scientific).

    Article Title: Tissue and Isoform-Specific Effects of Platelet-Derived Growth Factor on Neonatal-Derived Dermal and Fetal-Derived Lung Fibroblast Profibrotic Functions.
    Article Snippet: Approximately 7.5 μg of protein was loaded per well in a 4–20% MiniPROTEAN TGX Precast Protein Gel (cat. 4561096, Bio-Rad, Hercules, CA, USA). .. Protein was transferred to a 0.45 μm nitrocellulose membrane (cat. 1620115, Bio-Rad) and total protein was quantified with the Revert 700 Total Protein Stain Kit (cat. 926-11016, LI-COR Biotech), following the manufacturer’s instructions. .. Membranes were blocked for one hour at room temperature with Intercept Phosphate-Buffered Saline (PBS) Blocking Buffer (cat. https://doi.org/10.3390/cells15070637 927-70001, LI-COR Biotech) and probed overnight at 4 ◦C with anti-collagen-I antibody (1:1000 dilution, clone EPR7785, cat. ab138492, Abcam, Waltham, MA, USA), antifibronectin antibody (1:2000 dilution, clone IST-9, cat. sc-59826, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-smooth muscle actin polyclonal antibody (1:300 dilution, cat. PA5-85070, Thermo Fisher Scientific), anti-PDGFR alpha antibody (1:1000 dilution, clone EPR22059-270, cat. ab203491, Abcam), anti-PDGFR beta antibody (1:1000 dilution, clone 42G12, cat. ab69506, Abcam), anti-ERK1/ERK2 antibody (1:500 dilution, clone ERK-7D8, cat. 13-6200, Thermo Fisher Scientific), or anti-phospho-ERK1/2 antibody (1:1000 dilution, clone 2P8Q2, cat. MA5-38228, Thermo Fisher Scientific).

    Article Title: Co-Targeting Nuclear Export and Translation Initiation Uncovers a Therapeutic Vulnerability in Lethal Prostate Cancer
    Article Snippet: Proteins were then transferred to 0.2 μm nitrocellulose membranes using the Mini Trans-Blot® TurboTM semi-dry transfer system (Bio-Rad). .. Before blocking, a total protein stain was performed with the LICOR Revert 700 Total Protein Stain Kit; (Single-Color Western Blot 800 nm target only). ..

    Article Title: Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue Fibrosis
    Article Snippet: .. The blots were then stained for total protein using the Revert 700 Total Protein Stain Kit from LI-COR Biotech (Cat: 926-11016) according to the manufacturer’s protocol. .. After removing the Total Protein Stain, blots were blocked for 1 h in LI-COR Biotech Intercept Blocking Buffer (Cat: 927-60001) and probed with primary antibodies overnight at 4 °C.

    Membrane:

    Article Title: Jagged1 regulates extracellular matrix deposition and remodeling in triple-negative breast cancer
    Article Snippet: .. Cell lysates were directly spotted on a nitrocellulose membrane, allowed to dry, rinsed with MQ-H 2 O, and stained for total protein using the Revert 700 Total Protein Stain Kit (Li-COR) according to the manufacturer’s instructions. ..

    Article Title: Tissue and Isoform-Specific Effects of Platelet-Derived Growth Factor on Neonatal-Derived Dermal and Fetal-Derived Lung Fibroblast Profibrotic Functions
    Article Snippet: Approximately 7.5 μg of protein was loaded per well in a 4–20% Mini-PROTEAN TGX Precast Protein Gel (cat. 4561096, Bio-Rad, Hercules, CA, USA). .. Protein was transferred to a 0.45 μm nitrocellulose membrane (cat. 1620115, Bio-Rad) and total protein was quantified with the Revert 700 Total Protein Stain Kit (cat. 926-11016, LI-COR Biotech), following the manufacturer’s instructions. .. Membranes were blocked for one hour at room temperature with Intercept Phosphate-Buffered Saline (PBS) Blocking Buffer (cat. 927-70001, LI-COR Biotech) and probed overnight at 4 °C with anti-collagen-I antibody (1:1000 dilution, clone EPR7785, cat. ab138492, Abcam, Waltham, MA, USA), anti-fibronectin antibody (1:2000 dilution, clone IST-9, cat. sc-59826, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-smooth muscle actin polyclonal antibody (1:300 dilution, cat. PA5-85070, Thermo Fisher Scientific), anti-PDGFR alpha antibody (1:1000 dilution, clone EPR22059-270, cat. ab203491, Abcam), anti-PDGFR beta antibody (1:1000 dilution, clone 42G12, cat. ab69506, Abcam), anti-ERK1/ERK2 antibody (1:500 dilution, clone ERK-7D8, cat. 13-6200, Thermo Fisher Scientific), or anti-phospho-ERK1/2 antibody (1:1000 dilution, clone 2P8Q2, cat. MA5-38228, Thermo Fisher Scientific).

    Article Title: Tissue and Isoform-Specific Effects of Platelet-Derived Growth Factor on Neonatal-Derived Dermal and Fetal-Derived Lung Fibroblast Profibrotic Functions.
    Article Snippet: Approximately 7.5 μg of protein was loaded per well in a 4–20% MiniPROTEAN TGX Precast Protein Gel (cat. 4561096, Bio-Rad, Hercules, CA, USA). .. Protein was transferred to a 0.45 μm nitrocellulose membrane (cat. 1620115, Bio-Rad) and total protein was quantified with the Revert 700 Total Protein Stain Kit (cat. 926-11016, LI-COR Biotech), following the manufacturer’s instructions. .. Membranes were blocked for one hour at room temperature with Intercept Phosphate-Buffered Saline (PBS) Blocking Buffer (cat. https://doi.org/10.3390/cells15070637 927-70001, LI-COR Biotech) and probed overnight at 4 ◦C with anti-collagen-I antibody (1:1000 dilution, clone EPR7785, cat. ab138492, Abcam, Waltham, MA, USA), antifibronectin antibody (1:2000 dilution, clone IST-9, cat. sc-59826, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-smooth muscle actin polyclonal antibody (1:300 dilution, cat. PA5-85070, Thermo Fisher Scientific), anti-PDGFR alpha antibody (1:1000 dilution, clone EPR22059-270, cat. ab203491, Abcam), anti-PDGFR beta antibody (1:1000 dilution, clone 42G12, cat. ab69506, Abcam), anti-ERK1/ERK2 antibody (1:500 dilution, clone ERK-7D8, cat. 13-6200, Thermo Fisher Scientific), or anti-phospho-ERK1/2 antibody (1:1000 dilution, clone 2P8Q2, cat. MA5-38228, Thermo Fisher Scientific).

    Blocking Assay:

    Article Title: Co-Targeting Nuclear Export and Translation Initiation Uncovers a Therapeutic Vulnerability in Lethal Prostate Cancer
    Article Snippet: Proteins were then transferred to 0.2 μm nitrocellulose membranes using the Mini Trans-Blot® TurboTM semi-dry transfer system (Bio-Rad). .. Before blocking, a total protein stain was performed with the LICOR Revert 700 Total Protein Stain Kit; (Single-Color Western Blot 800 nm target only). ..

    Western Blot:

    Article Title: Co-Targeting Nuclear Export and Translation Initiation Uncovers a Therapeutic Vulnerability in Lethal Prostate Cancer
    Article Snippet: Proteins were then transferred to 0.2 μm nitrocellulose membranes using the Mini Trans-Blot® TurboTM semi-dry transfer system (Bio-Rad). .. Before blocking, a total protein stain was performed with the LICOR Revert 700 Total Protein Stain Kit; (Single-Color Western Blot 800 nm target only). ..



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